nis antibody conjugated with af488 (Novus Biologicals)

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Novus Biologicals nis antibody conjugated with af488
Nis Antibody Conjugated With Af488, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nis+antibody+conjugated+with+af488/SLC5A5%2FSodium+Iodide+Symporter+Antibody+(FP5)+%5BAlexa+Fluor%C2%AE+488%5D/pm35406554-82-32-52
Average 93 stars, based on 1 article reviews

nis antibody conjugated with af488 - by Bioz Stars, 2026-09

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Article Title: Targeting GLI1 Transcription Factor for Restoring Iodine Avidity with Redifferentiation in Radioactive-Iodine Refractory Thyroid Cancers
Article Snippet: After changing the culture medium to 500 μL Opti-MEM, the cells were treated with scrambled siRNA, GLI1 siRNA or NIS siRNA for 48 h. Subsequently, cells were washed three times with PBS and fixed using 4% paraformaldehyde (BIOSESANG) dissolved in 1X PBS for 10 min at RT. .. Thereafter, cells were rinsed three times with PBS and underwent the blocking step by using 3% BSA in PBS with Tween-20 (PBS-T) of 0.05% for 1 h. Cells were probed with both NIS antibody conjugated with AF488 (working dilution 1:250, wavelength excitation at 493 nm; emission at 517 nm, catalog number: NBP1-70342AF488, Novus Biologicals, Centennial, CO, USA) and sodium potassium ATPase (Na + /K + ATPase) antibody conjugated with AF647 (working dilution 1:250, wavelength excitation at 653 nm; emission at 668 nm, catalog number: ab198367, Abcam, Cambridge, United Kingdom) at 4 °C overnight. .. On the next day, the cells were washed with PBS three times and Hoechst 33342 (working dilution 1:2000 in 1X PBS, wavelength excitation at 348 nm; emission at 455 nm, catalog number: H3570, Thermo Fisher Scientific) added and incubated for 10 min at RT.

Article Title: Targeting GLI1 Transcription Factor for Restoring Iodine Avidity with Redifferentiation in Radioactive-Iodine Refractory Thyroid Cancers.
Article Snippet: After changing the culture medium to 500 μL Opti-MEM, the cells were treated with scrambled siRNA, GLI1 siRNA or NIS siRNA for 48 h. Subsequently, cells were washed three times with PBS and fixed using 4% paraformaldehyde (BIOSESANG) dissolved in 1X PBS for 10 min at RT. .. Thereafter, cells were rinsed three times with PBS and underwent the blocking step by using 3% BSA in PBS with Tween-20 (PBS-T) of 0.05% for 1 h. Cells were probed with both NIS antibody conjugated with AF488 (working dilution 1:250, wavelength excitation at 493 nm; emission at 517 nm, catalog number: NBP1-70342AF488, Novus Biologicals, Centennial, CO, USA) and sodium potassium ATPase (Na+/K+ ATPase) antibody conjugated with AF647 (working dilution 1:250, wavelength excitation at 653 nm; emission at 668 nm, catalog number: ab198367, Abcam, Cambridge, United Kingdom) at 4 ◦C overnight. .. On the next day, the cells were washed with PBS three times and Hoechst 33342 (working dilution 1:2000 in 1X PBS, wavelength excitation at 348 nm; emission at 455 nm, catalog number: H3570, Thermo Fisher Scientific) added and incubated for 10 min at RT.

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nis antibody conjugated with af488 (Novus Biologicals)

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